Optimization of Ultrasound-assisted Extraction of Phenolic Compounds from Robinia pseudoacacia L. Flowers by Response Surface Methodology

doi:10.7506/spkx1002-6630-201102016

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 66-70.doi: 10.7506/spkx1002-6630-201102016

• Processing Technology • Previous Articles Next Articles

Optimization of Ultrasound-assisted Extraction of Phenolic Compounds from Robinia pseudoacacia L. Flowers by Response Surface Methodology

WANG Xiao-yang，TANG Lin*，ZHAO Lei

College of Life Science, Shandong Normal University, Jinan 250014, China

Received:2010-03-05 Revised:2010-12-13 Online:2011-01-25 Published:2011-01-10
Contact: TANG Lin E-mail:tanglin@sdnu.edu.cn

Abstract

Abstract:

Objective: The optimization of the extraction process of phenolic compounds from Robinia pseudoacacia L.flowers under ultrasound assistance was achieved by response surface methodology to maximize phenolic extraction yield. Methods: On the basis of single-factor experiments, Box-Behnken central composite design (CCD) involving four variables at three levels was utilized to set up a quadratic polynomial regression equation for phenolic extraction yield as function of extraction temperature, ultrasound power, length of ultrasound treatment time and material/liquid ratio, and the pairwise interactive effects of the above four variables on their function were investigated by response surface analysis. Results: The optimum conditions for extracting phenolic compounds from Robinia pseudoacacia L.flowers were found to be: 36 min ultrasound treatment at 110 W power for extracting the raw material with a 20-fold volume of 60% aqueous ethanol solution at 35 ℃. Under these optimum conditions, the yield of polyphenols was 7.891%.

Key words: Robinia pseudoacacia L. flowers, phenolic compounds, response surface methodology

CLC Number:

TQ243.1

WANG Xiao-yang，TANG Lin*，ZHAO Lei. Optimization of Ultrasound-assisted Extraction of Phenolic Compounds from Robinia pseudoacacia L. Flowers by Response Surface Methodology[J]. FOOD SCIENCE, 2011, 32(2 ): 66-70.

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Optimization of Ultrasound-assisted Extraction of Phenolic Compounds from Robinia pseudoacacia L. Flowers by Response Surface Methodology

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